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BOS ISO 16649-3_2015
P376.20
Microbiology of food and animal feeding stuffs — Horizontal method for the detection and
enumeration of Listeria monocytogenes — Part 3:
Detection and most probable number technique
using 5-bromo-4-chloro-3-indolyl-ß-D-glucuronide
Description
This part of ISO 16649 specifies a horizontal method for the detection and enumeration of β-glucuronidase positive Escherichia coli, by means of the liquid-medium culture technique and
calculation of the most probable number (MPN) after incubation at (37 ± 1) °C, then at (44 ± 1) °C. This
part of ISO 16649 is applicable to the following:
— products intended for human consumption and the feeding of animals;
— environmental samples in the area of food production and food handling.
The method is suitable for the enumeration of cells of E. coli that might have been subjected to stress
arising from dehydration, freezing, and exposure to a saline (such as marine) environment or damage
by disinfectants such as chlorine-containing products.
A limitation of the applicability of this part of ISO 16649 is imposed by the susceptibility of the method
to a large degree of variability. The method is intended to be applied and the results interpreted in the
light of the information given in Clause 11.
This method has not been fully evaluated for all matrices (e.g. for milk and milk products). ISO 7251 is
intended to be used for milk and milk products.